Inflammatory mediators of experimental colitis in rats

Gastroenterology. 1989 Aug;97(2):326-37. doi: 10.1016/0016-5085(89)90068-1.


Colonic inflammation was induced in rats by intracolonic administration of 0.25 ml of 50% ethanol containing 30 mg of trinitrobenzene sulfonic acid (TNB). Control rats were treated with 0.25 ml of 50% ethanol or with 30 mg of TNB in 0.25 ml of saline. After 24 h, mucosal ulceration and hemorrhage were observed in TNB/ethanol-, 50% ethanol-, and to a lesser extent, in TNB/saline-treated rats. After 1 wk, mucosal damage was completely resolved in the 50% ethanol and TNB/saline-treated rats but the lesions in the TNB/ethanol-treated rats persisted and progressed to a chronic active inflammatory process after 3 wk. Myeloperoxidase activity was significantly elevated in mucosal scrapings from all treatment groups at all time intervals when macroscopic and microscopic mucosal injury was evident. Interleukin-1 was found to be the most sensitive indicator of mucosal inflammation, and its mucosal values correlated with myeloperoxidase activity. Leukotriene B4 was increased in control rats at 1 wk and in TNB/ethanol-treated rats at all time intervals. The maximal increase in leukotriene B4 was observed at 1 wk. Thromboxane B2 generation was reduced while platelet activating factor generation was not increased in TNB/ethanol-treated rats. These results indicate that in this TNB/ethanol model of gut inflammation, myeloperoxidase activity and interleukin-1 are reliable and sensitive indicators of colonic inflammation, and that thromboxane B2 is not involved in the acute lesions, whereas leukotriene B4 appears in the chronic active inflammatory response.

MeSH terms

  • Animals
  • Colitis / chemically induced
  • Colitis / metabolism*
  • Colon / pathology
  • Ethanol / toxicity
  • Interleukin-1 / metabolism
  • Intestinal Mucosa / pathology
  • Leukotriene B4 / metabolism
  • Male
  • Peroxidase / metabolism
  • Platelet Activating Factor / metabolism
  • Rats
  • Rats, Inbred Strains
  • Thromboxane B2 / metabolism
  • Trinitrobenzenesulfonic Acid / toxicity


  • Interleukin-1
  • Platelet Activating Factor
  • Leukotriene B4
  • Ethanol
  • Thromboxane B2
  • Trinitrobenzenesulfonic Acid
  • Peroxidase