Characterization and biological effects of two polysaccharides isolated from Acanthopanax sciadophylloides

Carbohydr Polym. 2015 Feb 13:116:159-66. doi: 10.1016/j.carbpol.2014.04.013. Epub 2014 Apr 19.


Two polysaccharides abbreviated ANP and AAP were isolated from the young buds of Acanthopanax sciadophylloides. ANP consisted of L-arabinose, D-mannose, D-glucose and D-galactose in a ratio of ca 1.0:2.6:2.5:1.4 and its weight average molecular weight (Mw) was 1.07×10(4). AAP consisted of L-arabinose, D-galactose and 4-O-methyl-D-glucuronic acid in a ratio of ca 5:10:1, and its Mw was estimated to be 8.40×10(4). ANP was suggested to be an acetylated heteropolysaccharide, whereas AAP was speculated to be a type II arabinogalactan on the basis of structural analysis data. Both polysaccharides were found to stimulate NO production and induce the expression of cytokine mRNAs including IL-1β, IL-6, IL-10 and TNF-α on RAW264.7 cells. They also induced NF-κB activation in RAW-Blue cells. NO production and NF-κB activation by both polysaccharides were decreased by pretreatment with neutralizing anti-TLR-4 and anti-CD14 antibodies but not with anti-TLR-2, anti-SR-A, anti-CD11c, and anti-Dectin-1 antibodies. Therefore, these immunostimulating effects of ANP and AAP were suggested to be promoted by the interaction through the membrane receptors, TLR-4 and CD14. In addition to immunomodulating effects, ANP showed anti-HSV-2 effects in vitro.

Keywords: Acanthopanax sciadophylloides; Anti-herpetic activity; Immunostimulation; Polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents* / chemistry
  • Antiviral Agents* / isolation & purification
  • Antiviral Agents* / pharmacology
  • Cell Line
  • Chlorocebus aethiops
  • Cytokines / genetics
  • Eleutherococcus / chemistry*
  • Female
  • Flowers / chemistry
  • Herpes Simplex / drug therapy
  • Herpesvirus 2, Human / drug effects*
  • Immunologic Factors* / chemistry
  • Immunologic Factors* / isolation & purification
  • Immunologic Factors* / pharmacology
  • Lipopolysaccharides
  • Mice, Inbred BALB C
  • NF-kappa B / metabolism
  • Nitric Oxide / metabolism
  • Phytotherapy
  • Polysaccharides* / chemistry
  • Polysaccharides* / isolation & purification
  • Polysaccharides* / pharmacology
  • RNA, Messenger / metabolism
  • Vero Cells


  • Antiviral Agents
  • Cytokines
  • Immunologic Factors
  • Lipopolysaccharides
  • NF-kappa B
  • Polysaccharides
  • RNA, Messenger
  • Nitric Oxide