Aptasensor for electrochemical sensing of angiogenin based on electrode modified by cationic polyelectrolyte-functionalized graphene/gold nanoparticles composites

Zhengbo Chen; Chenmeng Zhang; Xiaoxiao Li; He Ma; Chongqing Wan; Kai Li; Yuqing Lin

doi:10.1016/j.bios.2014.10.046

Aptasensor for electrochemical sensing of angiogenin based on electrode modified by cationic polyelectrolyte-functionalized graphene/gold nanoparticles composites

Biosens Bioelectron. 2015 Mar 15:65:232-7. doi: 10.1016/j.bios.2014.10.046. Epub 2014 Oct 22.

Authors

Zhengbo Chen¹, Chenmeng Zhang², Xiaoxiao Li², He Ma², Chongqing Wan³, Kai Li², Yuqing Lin²

Affiliations

¹ Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: czb979216@sina.com.
² Department of Chemistry, Capital Normal University, Beijing 100048, China.
³ Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: wancq@cnu.edu.cn.

PMID: 25461163
DOI: 10.1016/j.bios.2014.10.046

Abstract

Herein, a label-free and highly sensitive electrochemical aptasensor for the detection of angiogenin was proposed based on a conformational change of aptamer and amplification by poly(diallyldimethyl ammonium chloride) (PDDA)-functionalized graphene/gold nanoparticles (AuNPs) composites-modified electrode. PDDA-functionalized graphene (P-GR) nanosheets as the building block in the self-assembly of GR nanosheets/AuNPs heterostructure enhanced the electrochemical detection performance. The electrochemical aptasensor has an extraordinarily sensitive response to angiogenin in a linear range from 0.1pM to 5nM with a detection limit of 0.064pM. The developed sensor provides a promising strategy for the cancer diagnosis in medical application in the future.

Keywords: Angiogenin; Aptamer; Electrochemical; Gold nanoparticles; Graphene; Poly(diallyldimethyl ammonium chloride).

Publication types

Evaluation Study

MeSH terms

Allyl Compounds / chemistry
Aptamers, Nucleotide / chemistry*
Biosensing Techniques / economics
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Electrochemical Techniques / economics
Electrochemical Techniques / instrumentation
Electrochemical Techniques / methods
Equipment Design
Gold / chemistry*
Graphite / chemistry*
Humans
Limit of Detection
Metal Nanoparticles / chemistry
Metal Nanoparticles / ultrastructure
Nanocomposites / chemistry*
Nanocomposites / ultrastructure
Neoplasms / diagnosis
Polyamines / chemistry*
Polyelectrolytes
Quaternary Ammonium Compounds / chemistry
Ribonuclease, Pancreatic / analysis*

Substances

Allyl Compounds
Aptamers, Nucleotide
Polyamines
Polyelectrolytes
Quaternary Ammonium Compounds
polycations
Gold
Graphite
diallyldimethylammonium chloride
angiogenin
Ribonuclease, Pancreatic