Tetracycline-inducible gene expression system in Leishmania mexicana

Mol Biochem Parasitol. 2014 Nov;198(1):11-3. doi: 10.1016/j.molbiopara.2014.11.002. Epub 2014 Nov 22.


Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.

Keywords: Gene expression; Leishmania mexicana; Tet-inducible system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression*
  • Humans
  • Leishmania mexicana / drug effects*
  • Leishmania mexicana / genetics*
  • Leishmania mexicana / metabolism
  • Leishmaniasis, Cutaneous / parasitology*
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / metabolism
  • Tetracycline / pharmacology*


  • Protozoan Proteins
  • Tetracycline