Vegetable peptones increase production of type I collagen in human fibroblasts by inducing the RSK-CCAAT/enhancer binding protein-β phosphorylation pathway

Nutr Res. 2015 Feb;35(2):127-35. doi: 10.1016/j.nutres.2014.10.011. Epub 2014 Oct 30.


Skin aging appears to be principally attributed to a decrease in type I collagen level and the regeneration ability of dermal fibroblasts. We hypothesized that vegetable peptones promote cell proliferation and production of type I collagen in human dermal fibroblasts. Therefore, we investigated the effects of vegetable peptones on cell proliferation and type I collagen production and their possible mechanisms in human dermal fibroblasts. Vegetable peptones significantly promoted cell proliferation in a concentration-dependent manner. In addition, the human luciferase type I collagen α2 promoter and type I procollagen synthesis assays showed that the vegetable peptones induced type I procollagen production by activating the type I collagen α2 promoter. Moreover, the vegetable peptones activated p90 ribosomal s6 kinase, which was mediated by activating the Raf-p44/42 mitogen-activated protein kinase signaling pathway. Furthermore, the vegetable peptone-induced increase in cell proliferation and type I collagen production decreased upon treatment with the ERK inhibitor PD98059. Taken together, these findings suggest that increased proliferation of human dermal fibroblasts and enhanced production of type I collagen by vegetable peptones occur primarily by inducing the p90 ribosomal s6 kinase-CCAAT/enhancer binding protein β phosphorylation pathway, which is mediated by activating Raf-ERK signaling.

Keywords: Cell proliferation; Human dermal fibroblasts; RSK pathway; Skin aging; Type I procollagen; Vegetable peptones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCAAT-Enhancer-Binding Protein-beta / metabolism
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Collagen Type I / metabolism*
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Humans
  • Peptones / pharmacology*
  • Phosphorylation / drug effects
  • Promoter Regions, Genetic / drug effects
  • Ribosomal Protein S6 Kinases, 90-kDa / metabolism
  • Signal Transduction / drug effects*
  • Treatment Outcome
  • Vegetables / chemistry*


  • CCAAT-Enhancer-Binding Protein-beta
  • Collagen Type I
  • Peptones
  • Ribosomal Protein S6 Kinases, 90-kDa