Haemophilus influenzae surface fibril (Hsf) is a unique twisted hairpin-like trimeric autotransporter

Int J Med Microbiol. 2015 Jan;305(1):27-37. doi: 10.1016/j.ijmm.2014.10.004. Epub 2014 Oct 27.

Abstract

The Haemophilus surface fibril (Hsf) is an extraordinary large (2413 amino acids) trimeric autotransporter, present in all encapsulated Haemophilus influenzae. It contributes to virulence by directly functioning as an adhesin. Furthermore, Hsf recruits the host factor vitronectin thereby inhibiting the host innate immune response resulting in enhanced survival in serum. Here we observed by electron microscopy that Hsf appears as an 100 nm long fibril at the bacterial surface albeit the length is approximately 200 nm according to a bioinformatics based model. To unveil this discrepancy, we denaturated Hsf at the surface of Hib by using guanidine hydrochloride (GuHCl). Partial denaturation induced in the presence of GuHCl unfolded the Hsf molecules, and resulted in an increased length of fibres in comparison to the native trimeric form. Importantly, our findings were also verified by E. coli expressing Hsf at its surface. In addition, a set of Hsf-specific peptide antibodies also indicated that the N-terminal of Hsf is located near the C-terminal at the base of the fibril. Taken together, our results demonstrated that Hsf is not a straight molecule but is folded and doubled over. This is the first report that provides the unique structural features of the trimeric autotransporter Hsf.

Keywords: Haemophilus influenzae type b; Haemophilus surface fibril; Hib; Hsf.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / chemistry
  • Adhesins, Bacterial / genetics
  • Adhesins, Bacterial / metabolism*
  • Haemophilus influenzae / chemistry
  • Haemophilus influenzae / genetics
  • Haemophilus influenzae / metabolism*
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Microscopy, Electron
  • Models, Molecular
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / ultrastructure
  • Protein Conformation
  • Virulence Factors / chemistry
  • Virulence Factors / genetics
  • Virulence Factors / metabolism

Substances

  • Adhesins, Bacterial
  • Hsf protein, bacteria
  • Membrane Transport Proteins
  • Multiprotein Complexes
  • Virulence Factors