Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes

Food Chem. 2015 Apr 15;173:741-8. doi: 10.1016/j.foodchem.2014.10.067. Epub 2014 Oct 25.

Abstract

This study quantifies the bioactive molecules in and determines the in vitro protective effect of ethanolic extracts isolated from the peel and pulp of tucumã (Astrocaryum aculeatum, Mart.), an Amazonian fruit rich in carotenoids. The cytoprotective effect of tucumã was evaluated in lymphocyte cultures exposed to H2O2 using spectrophotometric, fluorimetric, and immunoassay assays. The results confirmed that tucumã pulp extract is rich in β-carotene and quercetin, as previously described in the literature. However, high levels of these compounds were also found in tucumã peel extract. The extracts also contained significant amounts rutin, gallic acid, caffeic acid, and chlorogenic acid. Despite quantitative differences in the concentration of these bioactive molecules, both extracts increased the viability of cells exposed to H2O2 in concentrations ranging from 300 to 900 μg/mL. Caspases 1, 3, and 8 decreased significantly in cells concomitantly exposed to H2O2 and these extracts, indicating that tucumã cryoprotection involves apoptosis modulation.

Keywords: Astrocaryum aculeatum; Carotenoids; DNA damage; Lymphocyte culture.

MeSH terms

  • Arecaceae / chemistry*
  • Caffeic Acids / analysis
  • Caffeic Acids / pharmacology
  • Caspase 1 / genetics
  • Caspase 1 / metabolism
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Caspase 8 / genetics
  • Caspase 8 / metabolism
  • Cell Survival / drug effects
  • Chlorogenic Acid / analysis
  • Chlorogenic Acid / pharmacology
  • Fruit / chemistry*
  • Gallic Acid / analysis
  • Gallic Acid / pharmacology
  • Humans
  • Hydrogen Peroxide / adverse effects*
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Plant Extracts / analysis
  • Plant Extracts / pharmacology*
  • Quercetin / analysis
  • Quercetin / pharmacology
  • Rutin / analysis
  • Rutin / pharmacology
  • beta Carotene / analysis
  • beta Carotene / pharmacology

Substances

  • Caffeic Acids
  • Plant Extracts
  • beta Carotene
  • Chlorogenic Acid
  • Rutin
  • Gallic Acid
  • Quercetin
  • Hydrogen Peroxide
  • CASP3 protein, human
  • CASP8 protein, human
  • Caspase 3
  • Caspase 8
  • Caspase 1
  • caffeic acid