The intake of broccoli sprouts modulates the inflammatory and vascular prostanoids but not the oxidative stress-related isoprostanes in healthy humans

Food Chem. 2015 Apr 15;173:1187-94. doi: 10.1016/j.foodchem.2014.10.152. Epub 2014 Nov 7.

Abstract

Current evidence supports the positive association between the consumption of plant foods and health. In this work, we assessed the effect of consuming a half-serving (30 g) or one serving (60 g) of broccoli sprouts on the urinary concentrations of biomarkers of oxidative stress (isoprostanes) and inflammation (prostaglandins and thromboxanes). Twenty-four volunteers participated in the project. A quantitative determination of sulforaphane and its mercapturic derivatives, eicosanoids, and total vitamin C in urine was performed. The intake of broccoli sprouts produced an increase in the urinary concentrations of sulforaphane metabolites and vitamin C. Among the 13 eicosanoids analyzed, tetranor-PGEM and 11β-PGF2α as well as 11-dehydro-TXB2 showed a significant decrease in their urinary concentrations after the ingestion of broccoli sprouts. Therefore, the consumption of broccoli sprouts modulated the excretion of biomarkers linked to inflammation and vascular reactions without exerting a significant influence on the oxidation of phospholipids in vivo.

Keywords: Broccoli; Eicosanoids; Oxidative stress; Sulforaphane; Urine; Vitamin C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Ascorbic Acid / pharmacokinetics
  • Ascorbic Acid / urine
  • Biomarkers / urine
  • Brassica / chemistry*
  • Chromatography, High Pressure Liquid
  • Cross-Over Studies
  • Female
  • Glucosinolates / pharmacokinetics
  • Glucosinolates / urine
  • Healthy Volunteers
  • Humans
  • Imidoesters / pharmacokinetics
  • Inflammation / prevention & control
  • Isoprostanes / pharmacokinetics
  • Isoprostanes / urine*
  • Isothiocyanates / pharmacokinetics
  • Isothiocyanates / urine
  • Male
  • Middle Aged
  • Oxidative Stress / drug effects*
  • Oximes
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacokinetics
  • Prostaglandins / pharmacokinetics
  • Prostaglandins / urine*
  • Sulfoxides
  • Tandem Mass Spectrometry
  • Thromboxane B2 / analogs & derivatives
  • Thromboxane B2 / pharmacokinetics
  • Thromboxane B2 / urine
  • Vascular Diseases / prevention & control
  • White People
  • Young Adult

Substances

  • Biomarkers
  • Glucosinolates
  • Imidoesters
  • Isoprostanes
  • Isothiocyanates
  • Oximes
  • Plant Extracts
  • Prostaglandins
  • Sulfoxides
  • tetranor prostaglandin M
  • Thromboxane B2
  • 11-dehydro-thromboxane B2
  • sulforaphane
  • Ascorbic Acid
  • glucoraphanin