A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase

Cell. 1989 Jul 28;58(2):409-19. doi: 10.1016/0092-8674(89)90855-6.

Abstract

A hyper-recombination mutation was isolated that causes an increase in recombination between short repeated delta sequences surrounding the SUP4-omicron gene in S. cerevisiae. The wild-type copy of this gene was cloned by complementation of one of its pleiotropic phenotypes, slow growth. DNA sequence of the clone revealed a 656 amino acid open reading frame capable of encoding a protein homologous to the bacterial type I topoisomerase. No homology was detected with previously identified eukaryotic topoisomerases. Construction of double mutants with either of the two known yeast topoisomerase genes revealed synergistic effects on growth suggesting overlapping functions. Expression of bacterial topoisomerase I in yeast can fully complement the slow growth defect of a null mutation. We have named this locus TOP3 and suggest that it defines a novel eukaryotic topoisomerase gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • DNA / analysis
  • DNA / genetics
  • DNA Topoisomerases, Type I / analysis
  • DNA Topoisomerases, Type I / genetics*
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Escherichia coli
  • Genetic Linkage
  • Molecular Sequence Data
  • Mutation*
  • Phenotype
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA, Bacterial
  • DNA
  • DNA Topoisomerases, Type I

Associated data

  • GENBANK/M24939