Vascular casting for the study of vascular morphogenesis

Methods Mol Biol. 2015;1214:49-66. doi: 10.1007/978-1-4939-1462-3_5.

Abstract

Microvascularity and angiogenesis play a pivotal role during normal growth and in a variety of pathological conditions such as inflammation, tumor growth, macular degeneration, and tissue regeneration. Vascular corrosion casting has been established as a method to analyze and evaluate two- and three-dimensionally the morphology and architecture of blood vessels of organs and tissues, such as tumors, brains, embryos, or the chorioallantoic membrane. Microvascular casts may be further dissected for visualizing and quantifying vascular morphology using scanning electron microscopy (SEM), micro computed tomographic (μCT) imaging, or synchrotron radiation-based micro computed tomographic (SRμCT) imaging.

MeSH terms

  • Adenocarcinoma / blood supply
  • Alzheimer Disease / physiopathology
  • Animals
  • Blood Vessels / growth & development*
  • Blood Vessels / physiology
  • Blood Vessels / physiopathology
  • Carcinoma, Squamous Cell / blood supply
  • Corrosion Casting / methods*
  • Humans
  • Mice
  • Molecular Imaging
  • Morphogenesis*
  • Neovascularization, Pathologic
  • Neovascularization, Physiologic
  • Regeneration
  • Vascular Remodeling