Alveolar bone regeneration in response to local application of calcitriol in vitamin D deficient rats

J Clin Periodontol. 2015 Jan;42(1):96-103. doi: 10.1111/jcpe.12342. Epub 2015 Jan 13.


Aim: Vitamin D deficiency is considered to diminish bone regeneration. Yet, raising the serum levels takes months. A topic application of the active vitamin D metabolite, calcitriol, may be an effective approach. Thus, it becomes important to know the effect of vitamin D deficiency and local application on alveolar bone regeneration.

Material and methods: Sixty rats were divided into three groups; two vitamin depletion groups and a control group. Identical single defects (2 mm diameter) were created in the maxilla and mandible treated with calcitriol soaked collagen in one deficiency group while in the other two groups not. Histomorphometric analysis and micro CTs were performed after 1 and 3 weeks. Serum levels of 25(OH)D3 and PTH were determined.

Results: Bone formation rate significantly increased within the observation period in all groups. Bone regeneration was higher in the maxilla than in the mandible. However, bone regeneration was lower in the control group compared to vitamin depletion groups, with no significant effects by local administration of calcitriol (micro CT mandible p = 0.003, maxilla p < 0.001; histomorphometry maxilla p = 0.035, mandible p = 0.18).

Conclusion: Vitamin D deficiency not necessarily impairs bone regeneration in the rat jaw and a single local calcitriol application does not enhance healing.

Keywords: 1,25(OH)D3; bone healing; calcitriol; supplementation; topic administration; vitamin D deficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Alveolar Bone Loss / drug therapy*
  • Alveolar Bone Loss / pathology
  • Alveolar Process / drug effects
  • Alveolar Process / pathology
  • Animals
  • Bone Density Conservation Agents / administration & dosage*
  • Bone Matrix / drug effects
  • Bone Matrix / pathology
  • Bone Regeneration / drug effects*
  • Calcifediol / blood
  • Calcification, Physiologic / drug effects
  • Calcitriol / administration & dosage*
  • Male
  • Mandibular Diseases / drug therapy
  • Mandibular Diseases / pathology
  • Maxillary Diseases / drug therapy
  • Maxillary Diseases / pathology
  • Organ Size
  • Osteoblasts / drug effects
  • Osteoblasts / pathology
  • Osteogenesis / drug effects
  • Parathyroid Hormone / blood
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors
  • Vitamin D Deficiency / complications*
  • X-Ray Microtomography / methods


  • Bone Density Conservation Agents
  • Parathyroid Hormone
  • Calcitriol
  • Calcifediol