Induction of protooncogene fos by extracellular signals in primary glial cell cultures

J Neurosci Res. 1989 Jun;23(2):234-9. doi: 10.1002/jnr.490230214.


In the present study various extracellular factors, acting through different second messenger systems, were examined for their capacity to increase the level of c-fos mRNA in primary glial cell cultures. In particular EGF, 12-O-tetradecanoylphorbol 13-acetate, the beta-adrenergic agonist isoproterenol, and the glutamate agonists, ibotenic and quisqualic acid, were studied. All the extracellular stimuli tested induced a rapid and transient increase in c-fos mRNA level in glial cell cultures regardless of the signal transduction pathway and the final effect on cell proliferation.

MeSH terms

  • Animals
  • Astrocytes / cytology
  • Astrocytes / drug effects
  • Astrocytes / metabolism*
  • Brain / cytology
  • Brain / metabolism
  • Cell Division / drug effects
  • Cells, Cultured
  • Cyclic AMP / metabolism
  • Cyclic AMP / physiology*
  • DNA-Binding Proteins / metabolism*
  • Epidermal Growth Factor / pharmacology*
  • Ibotenic Acid / pharmacology
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger / metabolism*
  • Rats
  • Second Messenger Systems / drug effects*
  • Tetradecanoylphorbol Acetate / pharmacology*


  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger
  • Ibotenic Acid
  • Epidermal Growth Factor
  • Cyclic AMP
  • Tetradecanoylphorbol Acetate