Abstract
In the present study various extracellular factors, acting through different second messenger systems, were examined for their capacity to increase the level of c-fos mRNA in primary glial cell cultures. In particular EGF, 12-O-tetradecanoylphorbol 13-acetate, the beta-adrenergic agonist isoproterenol, and the glutamate agonists, ibotenic and quisqualic acid, were studied. All the extracellular stimuli tested induced a rapid and transient increase in c-fos mRNA level in glial cell cultures regardless of the signal transduction pathway and the final effect on cell proliferation.
MeSH terms
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Animals
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Astrocytes / cytology
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Astrocytes / drug effects
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Astrocytes / metabolism*
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Brain / cytology
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Brain / metabolism
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Cell Division / drug effects
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Cells, Cultured
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Cyclic AMP / metabolism
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Cyclic AMP / physiology*
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DNA-Binding Proteins / metabolism*
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Epidermal Growth Factor / pharmacology*
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Ibotenic Acid / pharmacology
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-fos
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RNA, Messenger / metabolism*
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Rats
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Second Messenger Systems / drug effects*
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Tetradecanoylphorbol Acetate / pharmacology*
Substances
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DNA-Binding Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fos
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RNA, Messenger
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Ibotenic Acid
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Epidermal Growth Factor
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Cyclic AMP
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Tetradecanoylphorbol Acetate