In vitro adhesion of oral Candida dubliniensis isolates to acrylic denture surfaces following brief exposure to sub-cidal concentrations of polyenes, azoles and chlorhexidine

Med Princ Pract. 2015;24(1):58-64. doi: 10.1159/000369019. Epub 2014 Nov 29.

Abstract

Objectives: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures.

Methods: After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay.

Results: Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively.

Conclusions: Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Anti-Infective Agents, Local / pharmacology*
  • Antifungal Agents / pharmacology
  • Azoles / administration & dosage
  • Azoles / pharmacology*
  • Candida / drug effects*
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / pharmacology*
  • Dental Clinics
  • Dentures / microbiology*
  • Humans
  • Kuwait
  • Microbial Sensitivity Tests
  • Polyenes / administration & dosage
  • Polyenes / pharmacology*
  • Polymethyl Methacrylate

Substances

  • Anti-Infective Agents, Local
  • Antifungal Agents
  • Azoles
  • Polyenes
  • Polymethyl Methacrylate
  • Chlorhexidine