Characterization of exopolysaccharide and ropy capsular polysaccharide formation by Weissella

Saskia Katharina Malang; Ndegwa Henry Maina; Clarissa Schwab; Maija Tenkanen; Christophe Lacroix

doi:10.1016/j.fm.2014.08.022

Characterization of exopolysaccharide and ropy capsular polysaccharide formation by Weissella

Food Microbiol. 2015 Apr:46:418-427. doi: 10.1016/j.fm.2014.08.022. Epub 2014 Sep 7.

Authors

Saskia Katharina Malang¹, Ndegwa Henry Maina², Clarissa Schwab³, Maija Tenkanen⁴, Christophe Lacroix⁵

Affiliations

¹ Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, 8092, Zürich, Switzerland. Electronic address: saskia.malang@hest.ethz.ch.
² Department of Food and Environmental Sciences, P.O. Box 27, FI-00014, University of Helsinki, Finland. Electronic address: henry.maina@helsinki.fi.
³ Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, 8092, Zürich, Switzerland. Electronic address: clarissa.schwab@hest.ethz.ch.
⁴ Department of Food and Environmental Sciences, P.O. Box 27, FI-00014, University of Helsinki, Finland. Electronic address: maija.tenkanen@helsinki.fi.
⁵ Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, 8092, Zürich, Switzerland. Electronic address: christophe.lacroix@hest.ethz.ch.

PMID: 25475311
DOI: 10.1016/j.fm.2014.08.022

Abstract

With their broad functional properties, lactic acid bacteria derived high molar mass exopolysaccharides (EPS) and oligosaccharides are of great interest for food, medical and pharmaceutical industry. EPS formation by 123 strains of Weissella cibaria and Weissella confusa, was evaluated. Dextran formation from sucrose was observed for all tested strains while 18 strains produced fructan in addition to dextran. Six isolates synthesized a highly ropy polymer from glucose associated with the formation of a cell-bound, capsular polysaccharide (CPS) composed of glucose, O-acetyl groups and two unidentified monomer components. The soluble EPSs of nine strains were identified as low α-1,3-branched dextran, levan and inulin type polymers using NMR. In addition to glucan and fructan, W. confusa produced gluco- and fructooligosaccharides. Partial dextransucrase and fructansucrase sequences were characterized in the selected Weissella strains. Our study reports the first structural characterization of fructan type EPS from Weissella as well as the first Weissella strain producing inulin. Production of more than one EPS-type by single strains may have high potential for development of applications combining EPS technological and nutritional benefits.

Keywords: Capsular polysaccharide; Dextran; Exopolysaccharide; Inulin; Levan; Weissella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Capsules / chemistry*
Bacterial Capsules / metabolism*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Weissella / chemistry
Weissella / classification
Weissella / genetics
Weissella / metabolism*

Substances

Bacterial Proteins