A role for peroxidasin PXN-1 in aspects of C. elegans development

Mol Cells. 2015 Jan 31;38(1):51-7. doi: 10.14348/molcells.2015.2202. Epub 2014 Dec 4.


The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.

Keywords: C. elegans; heat stress; neuronal development; peroxidasins; pxn-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism*
  • Extracellular Matrix / metabolism
  • Extracellular Matrix Proteins / genetics*
  • Extracellular Matrix Proteins / metabolism*
  • Genes, Lethal
  • Hot Temperature
  • Mutation
  • Neurons / metabolism*
  • Peroxidase / genetics*
  • Peroxidase / metabolism*
  • Peroxiredoxins / genetics*
  • Peroxiredoxins / metabolism*


  • Caenorhabditis elegans Proteins
  • Extracellular Matrix Proteins
  • PXN-1 protein, C elegans
  • peroxidasin
  • Peroxiredoxins
  • Peroxidase