Evaluation of tests to predict metallo-β-lactamase in cystic fibrosis (CF) and non-(CF) Pseudomonas

Braz J Microbiol. 2014 Oct 9;45(3):835-9. doi: 10.1590/s1517-83822014000300011. eCollection 2014.

Abstract

Double disks synergy test (DDST) and combined disks test (CD) were evaluated to predict the presence of metallo-β-lactamase in 70 Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients. DDST(CAZ-EDTA 1 cm) and CD(IMP-EDTA) tests showed the best accuracy (94.3%). Furthermore, for other combinations, accuracy unsatisfactory was obtained.

Keywords: Pseudomonas aeruginosa; metallo-β-lactamase; phenotypic tests.

Publication types

  • Evaluation Study

MeSH terms

  • Cystic Fibrosis / complications
  • Humans
  • Microbial Sensitivity Tests / methods
  • Phenotype
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / isolation & purification
  • Respiratory Tract Infections / microbiology*
  • beta-Lactamases / metabolism*

Substances

  • beta-Lactamases