Desulfovibrio Desulfuricans Isolates From the Gut of a Single Individual: Structural and Biological Lipid A Characterization

FEBS Lett. 2015 Jan 2;589(1):165-71. doi: 10.1016/j.febslet.2014.11.042. Epub 2014 Dec 3.

Abstract

The levels of sulfate-reducing bacteria (SRB), including Desulfovibrionaceae, in the gut increase following a fat-enriched diet. Endotoxins from gut microbiota contribute to the inflammation process, leading to metabolic diseases. Thus, we sought to characterize the lipid A structures of Desulfovibrionaceae lipopolysaccharides (LPS) that are associated with the microbiota inflammatory properties. LPS variants were obtained from two SRB isolates from the gut of a single individual. These LPS variants shared similar lipid A moieties with Enterobacterial LPS, but differed from one another with regard to fatty-acid numbers and endotoxic activity. This first complete structural characterization of Desulfovibrio lipid A gives new insights into previously published data on Desulfovibrio lipid A biosynthesis. LPS microdiversity within SRBs illustrates how adaptation can influence pro-inflammatory potential.

Keywords: Cytokine; Desulfovibrionaceae; Endotoxin; Inflammation; Lipid A; Mass spectrometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Desulfovibrio desulfuricans / chemistry*
  • Desulfovibrio desulfuricans / isolation & purification
  • Desulfovibrio desulfuricans / metabolism
  • Humans
  • Intestines / microbiology*
  • Lipid A / chemistry*
  • Lipid A / metabolism
  • Lipid A / toxicity

Substances

  • Lipid A