New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood

PLoS One. 2014 Dec 5;9(12):e113052. doi: 10.1371/journal.pone.0113052. eCollection 2014.

Abstract

Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / genetics*
  • Cell Line
  • Cellular Reprogramming / genetics
  • Gene Expression
  • Genetic Vectors*
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Pan troglodytes
  • Sendai virus / genetics*
  • Transduction, Genetic*
  • Transgenes

Grant support

This study was supported in part by grants from the Ministry of Health, Labor, and Welfare of Japan, Precursory Research for Embryonic Science and Technology (PRESTO), and Core Research for Evolutional Science and Technology (CREST), Japan Science and Project of the Primate Research Institute, Kyoto University. DNAVEC Corporation provided support in the form of salaries for authors MH & HB, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NF was provided with salary by JST grant (PRESTO). The specific roles of these authors are articulated in the 'author contributions' section.