Novel TNS3-MAP3K3 and ZFPM2-ELF5 fusion genes identified by RNA sequencing in multicystic mesothelioma with t(7;17)(p12;q23) and t(8;11)(q23;p13)

Cancer Lett. 2015 Feb 28;357(2):502-9. doi: 10.1016/j.canlet.2014.12.002. Epub 2014 Dec 4.


Multicystic mesothelioma is a rare disease of unknown etiology and pathogenesis. Nothing has been known about the cytogenetic and molecular genetic features of these tumors. Here we present the first cytogenetically analyzed multicystic mesothelioma with the karyotype 46,XX,t(7;17)(p13;q23),t(8;11)(q23;p13). RNA-sequencing showed that the t(7;17)(p13;q23) generated a chimeric TNS3-MAP3K3 gene, which codes for a chimeric protein kinase, as well as the reciprocal MAP3K3-TNS3 in which the region of TNS3 coding for the SH2_Tensin_like region and the tensin phosphotyrosine-binding domain is under the control of the MAP3K3 promoter. The other translocation, t(8;11)(q23;p13), generated a chimeric ZFPM2-ELF5 gene which codes for a chimeric transcription factor in which the first 40 amino acids of ELF5 are replaced by the first 100 amino acids of ZFPM2. RT-PCR together with Sanger sequencing verified the presence of the above-mentioned fusion transcripts. The finding of acquired clonal chromosome abnormalities in cells cultured from the lesion and the presence of the TNS3-MAP3K3 chimeric protein kinase and the ZFPM2-ELF5 chimeric transcription factor confirm the neoplastic nature of multicystic mesothelioma.

Keywords: Chimeric protein kinase; Chimeric transcription factor; Cytogenetics; Fusion genes; Multicystic mesothelioma; RNA sequencing.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Chromosome Banding
  • Chromosomes, Human, Pair 11 / genetics
  • Chromosomes, Human, Pair 17 / genetics
  • Chromosomes, Human, Pair 7 / genetics
  • Chromosomes, Human, Pair 8 / genetics
  • DNA-Binding Proteins / genetics*
  • Diagnosis, Differential
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotype
  • MAP Kinase Kinase Kinase 3 / genetics*
  • Mesothelioma, Cystic / diagnosis
  • Mesothelioma, Cystic / genetics*
  • Mesothelioma, Cystic / surgery
  • Microfilament Proteins / genetics*
  • Molecular Sequence Data
  • Oncogene Proteins, Fusion / genetics
  • Proto-Oncogene Proteins c-ets / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, RNA / methods
  • Tensins
  • Transcription Factors / genetics*
  • Translocation, Genetic*


  • DNA-Binding Proteins
  • ELF5 protein, human
  • Microfilament Proteins
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Proteins c-ets
  • TNS3 protein, human
  • TNS3-MAP3K3 fusion protein, human
  • Tensins
  • Transcription Factors
  • ZFPM2 protein, human
  • ZFPM2-ELF5 fusion protein, human
  • MAP Kinase Kinase Kinase 3
  • MAP3K3 protein, human