Purification, Crystallization and Preliminary X-ray Crystallographic Studies of KstR2 (Ketosteroid Regulatory Protein) From Mycobacterium Tuberculosis

Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1643-5. doi: 10.1107/S2053230X14023589. Epub 2014 Nov 14.

Abstract

KstR2 (Rv3557c) is one of two TetR-family transcriptional repressors of cholesterol metabolism in Mycobacterium tuberculosis. The ability to degrade cholesterol fully is important for pathogenesis, and therefore this repressor was expressed, purified and crystallized. Crystals of KstR2 diffracted to better than 1.9 Å resolution and belonged to space group C2, with unit-cell parameters a = 72.3, b = 90.3, c = 49.7 Å, α = γ = 90, β = 128.2°.

Keywords: Mycobacterium tuberculosis; TetR family; cholesterol; steroid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / isolation & purification
  • Crystallization
  • Crystallography, X-Ray / methods*
  • Mycobacterium tuberculosis / chemistry*

Substances

  • Bacterial Proteins