Characterization of 17 Strains Belonging to the Mycobacterium Simiae Complex and Description of Mycobacterium Paraense Sp. Nov

Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):656-662. doi: 10.1099/ijs.0.068395-0. Epub 2014 Dec 8.

Abstract

Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Pará (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the M. simiae complex, being most closely related to Mycobacterium interjectum. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus Mycobacterium, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26(T) = DSM 46749(T) = CCUG 66121(T)). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.

MeSH terms

  • Bacterial Typing Techniques
  • Brazil
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Humans
  • Italy
  • Molecular Sequence Data
  • Mycobacterium / classification*
  • Mycobacterium / genetics
  • Mycobacterium / isolation & purification
  • Mycobacterium Infections / microbiology*
  • Mycolic Acids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sputum / microbiology

Substances

  • DNA, Bacterial
  • Mycolic Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EU370529
  • GENBANK/HM056136
  • GENBANK/HM056137
  • GENBANK/HM056138
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