Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 30 (12), 1157-63

Fingerprint Analysis and Multi-Ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

Affiliations

Fingerprint Analysis and Multi-Ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

Huali Liao et al. Anal Sci.

Abstract

A method employing high performance liquid chromatography-diode array detection (HPLC-DAD) has been developed to evaluate the quality of Saposhnikoviae Radix (SR) comprehensively through fingerprint analysis and multi-ingredient determination. In the fingerprint analysis, 12 out of 15 common peaks were identified with high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) and related references. To discriminate the 15 batches of samples collected from different origins, similarity analysis and principal component analysis (PCA) were performed. Meanwhile, the simultaneous quantification of four chromones was achieved by firstly applying a method of using a single standard to determine multiple components (SSDMC) with conversion factor. The method has the advantage of feasibility, economy and simplicity compared with traditional external standard method (ESM). The method possessed desirable linearity (R(2) ≥ 0.9997), precision (RSD <4.0%), accuracy (97.4 - 106.6%) and robustness. This study indicated that the method of fingerprint analysis integrated with multi-ingredient determination using the HPLC technique was reliable for the overall quality evaluation of SR.

Similar articles

See all similar articles

Cited by 4 PubMed Central articles

Publication types

MeSH terms

Substances

Feedback