Induced transposition of Ds by a stable Ac in crosses of transgenic tobacco plants

Mol Gen Genet. 1989 May;217(1):53-9. doi: 10.1007/BF00330942.

Abstract

A two component maize transposon system comprising a stable but trans-active Ac element and a cis responsive Ds element has been established in transgenic tobacco. The development of this system is desirable for the realization of a gene tagging strategy employing these elements in heterologous plant species. Transgenic tobacco with a single transposed Ac element (Ac-18) which has sustained a 4 bp terminal deletion has been identified. Transposase activity of the stable Ac-18 was demonstrated in a tissue culture assay as well as in transgenic plants. When plants containing Ac-18 were crossed with transgenic plants carrying a Ds element, 25%-50% of the F1 progeny showed trans-activation of Ds transposition. Analysis of DNA of several F1 plants showed that each plant displayed a unique pattern of Ds transposition to new chromosomal sites.

MeSH terms

  • Blotting, Southern
  • Chromosome Deletion
  • Cloning, Molecular
  • Crosses, Genetic*
  • DNA / genetics
  • DNA Transposable Elements*
  • Drug Resistance / genetics
  • Kanamycin / pharmacology
  • Nicotiana / genetics*
  • Nucleotidyltransferases / metabolism
  • Plants, Toxic*
  • Restriction Mapping
  • Transformation, Genetic
  • Transposases
  • Zea mays / genetics*

Substances

  • DNA Transposable Elements
  • Kanamycin
  • DNA
  • Nucleotidyltransferases
  • Transposases