Pyroglutamic acid stimulates DNA synthesis in rat primary hepatocytes through the mitogen-activated protein kinase pathway

Biosci Biotechnol Biochem. 2015;79(5):795-8. doi: 10.1080/09168451.2014.991689. Epub 2014 Dec 15.

Abstract

We purified pyroglutamic acid from human placental extract and identified it as a potent stimulator of rat primary hepatocyte DNA synthesis. Pyroglutamic acid dose-dependently stimulated DNA synthesis, and this effect was inhibited by PD98059, a dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) inhibitor. Therefore, pyroglutamic acid stimulated DNA synthesis in rat primary hepatocytes via MAPK signaling.

Keywords: DNA synthesis; mitogen-activated protein kinase; placental extracts; pyroglutamic acid; rat primary hepatocyte.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromatography, Liquid / methods
  • DNA / biosynthesis*
  • DNA / drug effects
  • Female
  • Flavonoids / pharmacology
  • Hepatocytes / drug effects*
  • Hepatocytes / physiology
  • Humans
  • Imidazoles / pharmacology
  • MAP Kinase Signaling System / drug effects*
  • Male
  • Mass Spectrometry / methods
  • Placenta / chemistry
  • Pregnancy
  • Protein Kinase Inhibitors / pharmacology
  • Pyridines / pharmacology
  • Pyrrolidonecarboxylic Acid / isolation & purification
  • Pyrrolidonecarboxylic Acid / pharmacology*
  • Rats, Sprague-Dawley

Substances

  • Flavonoids
  • Imidazoles
  • Protein Kinase Inhibitors
  • Pyridines
  • DNA
  • SB 203580
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
  • Pyrrolidonecarboxylic Acid