Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase

Elife. 2014 Dec 15;3:e04506. doi: 10.7554/eLife.04506.

Abstract

Histone modifiers play essential roles in controlling transcription and organizing eukaryotic genomes into functional domains. Here, we show that Set1, the catalytic subunit of the highly conserved Set1C/COMPASS complex responsible for histone H3K4 methylation (H3K4me), behaves as a repressor of the transcriptome largely independent of Set1C and H3K4me in the fission yeast Schizosaccharomyces pombe. Intriguingly, while Set1 is enriched at highly expressed and repressed loci, Set1 binding levels do not generally correlate with the levels of transcription. We show that Set1 is recruited by the ATF/CREB homolog Atf1 to heterochromatic loci and promoters of stress-response genes. Moreover, we demonstrate that Set1 coordinates with the class II histone deacetylase Clr3 in heterochromatin assembly at prominent chromosomal landmarks and repression of the transcriptome that includes Tf2 retrotransposons, noncoding RNAs, and regulators of development and stress-responses. Our study delineates a molecular framework for elucidating the functional links between transcriptome control and chromatin organization.

Keywords: HDAC; S. pombe; atf1; chromatin; chromosomes; evolutionary biology; genes; genomics; heterochromatin; set1; transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activating Transcription Factor 1 / genetics
  • Activating Transcription Factor 1 / metabolism
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Chromosomes, Fungal / chemistry*
  • Gene Expression Regulation, Fungal*
  • Heterochromatin / chemistry*
  • Heterochromatin / metabolism
  • Histone-Lysine N-Methyltransferase / genetics*
  • Histone-Lysine N-Methyltransferase / metabolism
  • Histones / genetics
  • Histones / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Promoter Regions, Genetic
  • RNA, Fungal / genetics*
  • RNA, Fungal / metabolism
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces pombe Proteins / genetics*
  • Schizosaccharomyces pombe Proteins / metabolism
  • Signal Transduction
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcriptome

Substances

  • Activating Transcription Factor 1
  • Cell Cycle Proteins
  • Clr3 protein, S pombe
  • Heterochromatin
  • Histones
  • Phosphoproteins
  • RNA, Fungal
  • RNA, Untranslated
  • Schizosaccharomyces pombe Proteins
  • Tf2-6 protein, S pombe
  • Transcription Factors
  • atf1 protein, S pombe
  • Histone-Lysine N-Methyltransferase
  • Set1 protein, S pombe

Associated data

  • GEO/GSE63301

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.