A newly developed kit for the measurement of urinary liver-type fatty acid-binding protein as a biomarker for acute kidney injury in patients with critical care

J Infect Chemother. 2015 Mar;21(3):165-9. doi: 10.1016/j.jiac.2014.10.017. Epub 2014 Nov 18.

Abstract

In recent years, it has been reported that the urinary level of Liver-type fatty acid-binding protein (L-FABP) serves as a useful biomarker for diagnosing acute kidney injury (AKI) or sepsis complicated by AKI. However, because the urinary level of L-FABP is currently measured by enzyme-linked immunosorbent assay (ELISA), several days may elapse before the results of the measurement become available. We have newly developed a simplified kit, the Dip-test, for measuring the urinary level of L-FABP. The Dip-test was measured at 80 measurement points (22 points in noninfectious disease, 13 points in SIRS, 20 points in infectious disease, and 25 points in sepsis) in 20 patients. The urinary L-FABP levels as determined by ELISA in relation to the results of the Dip-test were as follows: 10.10 ± 12.85 ng/ml in patients with a negative Dip-test ([-] group), 41.93 ± 50.51 ng/ml in patients with a ± test ([±] group), 70.36 ± 73.70 ng/ml in patients with a positive test ([+] group), 1048.96 ± 2117.68 ng/ml in patients with a 2 + test ([2+] group), and 23,571.55 ± 21,737.45 ng/ml in patients with a 3 + test ([3+] group). The following tendency was noted: the stronger the positive Dip-test reaction, the higher the urinary L-FABP level. Multigroup comparison revealed a significant differences in the urinary L-FABP levels between the Dip-test (-) group and each of the other groups. In this study, the usefulness of the Dip-test, our newly developed simplified kit for measuring the urinary L-FABP level, is suggested.

Keywords: Dip-test; Enzyme-linked immunosorbent assay; Point of care testing; Urinary liver-type fatty acid-binding protein.

Publication types

  • Observational Study

MeSH terms

  • Acute Kidney Injury / urine*
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / urine*
  • Critical Care
  • Enzyme-Linked Immunosorbent Assay
  • Fatty Acid-Binding Proteins / urine*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Point-of-Care Systems
  • Prospective Studies
  • Reagent Kits, Diagnostic

Substances

  • Biomarkers
  • FABP1 protein, human
  • Fatty Acid-Binding Proteins
  • Reagent Kits, Diagnostic