Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences

Eur J Biochem. 1989 Aug 15;183(3):603-10. doi: 10.1111/j.1432-1033.1989.tb21089.x.


A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted protein sequence is 92% identical (94% similar) to rabbit protein phosphatase 1 alpha (PP-1 alpha) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1 alpha, proves the existence of at least two transcriptionally active genes for protein phosphatase 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution*
  • Chromosome Mapping*
  • Cloning, Molecular*
  • DNA / genetics*
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics*
  • Genes
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Phosphoprotein Phosphatases / genetics*
  • Protein Biosynthesis
  • Protein Phosphatase 1
  • Rabbits / genetics
  • Salivary Glands / enzymology
  • Sequence Homology, Nucleic Acid
  • X Chromosome


  • Macromolecular Substances
  • Oligonucleotide Probes
  • DNA
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1

Associated data

  • GENBANK/X15583