The role of sodium ions in methanogenesis. Formaldehyde oxidation to CO2 and 2H2 in methanogenic bacteria is coupled with primary electrogenic Na+ translocation at a stoichiometry of 2-3 Na+/CO2

Eur J Biochem. 1989 Sep 1;184(1):223-32. doi: 10.1111/j.1432-1033.1989.tb15010.x.

Abstract

Cell suspensions of Methanosarcina barkeri were found to oxidize formaldehyde to CO2 and 2H2 (delta G0' = -27 kJ/mol CO2), when methanogenesis was inhibited by 2-bromoethanesulfonate. We report here that this reaction is coupled with (a) primary electrogenic Na+ translocation at a stoichiometry of 2-3 Na+/CO2, (b) with secondary H+ translocation via a Na+/H+ antiporter and (c) with ATP synthesis driven by an electrochemical proton potential. This is concluded from the following findings. Formaldehyde oxidation to CO2 and 2H2 was dependent on Na+ ions, 2-3 mol Na+/mol formaldehyde oxidized were extruded. Na+ translocation was inhibited by Na+ ionophores, but not affected by protonophores of Na+/H+ antiport inhibitors. Formaldehyde oxidation was associated with the build up of a membrane potential in the order of 100 mV (inside negative), which could be dissipated by sodium ionophores rather than by protonophores. Formaldehyde oxidation was coupled with ATP synthesis, which could be inhibited by Na+ ionophores, Na+/H+ antiport inhibitors, by protonophores and by the H+-translocating-ATP-synthase inhibitor, dicyclohexylcarbodiimide. With cell suspensions of Methanobacterium thermoautotrophicum similar results were obtained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Alkanesulfonates / pharmacology
  • Alkanesulfonic Acids*
  • Biological Transport, Active
  • Carbon Dioxide / metabolism*
  • Carrier Proteins / metabolism
  • Euryarchaeota / drug effects
  • Euryarchaeota / metabolism*
  • Formaldehyde / metabolism*
  • Kinetics
  • Models, Theoretical
  • Oxidation-Reduction
  • Proton-Translocating ATPases / metabolism
  • Sodium / metabolism*
  • Sodium-Hydrogen Exchangers

Substances

  • Alkanesulfonates
  • Alkanesulfonic Acids
  • Carrier Proteins
  • Sodium-Hydrogen Exchangers
  • Carbon Dioxide
  • Formaldehyde
  • 2-bromoethanesulfonic acid
  • Adenosine Triphosphate
  • Sodium
  • Proton-Translocating ATPases