Cytotoxic effect of 8-amino adenosine 3',5'-cyclic monophosphate on FM3A mouse mammary tumor cells

J Pharmacobiodyn. 1989 Jun;12(6):357-62. doi: 10.1248/bpb1978.12.357.


Derivatives of adenosine 3',5'-cyclic monophosphate (cAMP) with modifications at the 8-position were synthesized and examined for their cytotoxic effects on FM3A mouse mammary tumor cells and ZR-75 human mammary tumor cells. On in vitro tests of these derivatives, 8-amino (8-NH2) cAMP was the most effective against both cell lines. This compound showed the dose-dependent inhibition of FM3A cell growth in the concentration of over 2.5 microM with the ID50 value of 4 microM. Furthermore, antitumor activity of 8-NH2 cAMP was also tested against FM3A cells in vivo. T/C% values of 8-NH2 cAMP were respectively 162% and 138% in response to doses of 30 and 10 mg/kg by intraperitoneal injections of 8-NH2 cAMP for 5 d. 8-NH2 cAMP was converted to 8-NH2 adenosine via 8-NH2 adenosine 5'-monophosphate by some enzymes in the fetal bovine serum and the cytotoxic effect of 8-NH2 cAMP on FM3A cells was actually stemmed from 8-NH2 adenosine.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antineoplastic Agents*
  • Chromatography, High Pressure Liquid
  • Cyclic AMP / analogs & derivatives*
  • Cyclic AMP / pharmacology
  • Dipyridamole / pharmacology
  • Female
  • Humans
  • Mammary Neoplasms, Experimental / drug therapy*
  • Mice
  • Tumor Cells, Cultured


  • Antineoplastic Agents
  • Dipyridamole
  • Cyclic AMP