Regulatory elements involved in the tissue-specific expression of the yellow gene of Drosophila

Mol Gen Genet. 1989 Jul;218(1):118-26. doi: 10.1007/BF00330574.


We have assessed the DNA sequence requirements for the correct spatial pattern and phenotypic expression of y in the late embryo/larvae. The wild-type larval phenotype requires both the regions between -294 bp and -92 bp and a portion of the intron; the sequence element(s) located within the intron can act in a position independent manner to effect the wild-type larval phenotype. The larval expression pattern was examined by tissue experiments in situ and by staining germline transformants derived from various y/lacZ fusion constructs. The larval expression of y is restricted to the mouthparts, microsetae and anal plates. While the -495 bp to +194 bp region alone cannot effect a wild-type larval expression pattern, this region in conjunction with the intron appears to be sufficient to drive beta-gal expression in an essentially wild-type pattern. Our data further suggest that the -294 bp to -92 bp region contains elements which specify the larval pattern and that the element(s) in the intron normally act to enhance the level of expression necessary for the wild-type larval phenotype. We also present a phenotypic analysis of the adult cuticle structures of germline transformants derived from a variety of deletion and rearrangement constructs of the y gene. This analysis has revealed several new features associated with the regulation of y expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Transposable Elements
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila Proteins*
  • Gene Expression Regulation
  • Insect Hormones / biosynthesis
  • Insect Hormones / genetics*
  • Introns
  • Lac Operon
  • Nucleic Acid Hybridization
  • Phenotype
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid*
  • Restriction Mapping
  • Transcription, Genetic
  • Transformation, Genetic
  • beta-Galactosidase / metabolism


  • DNA Transposable Elements
  • Drosophila Proteins
  • Insect Hormones
  • y protein, Drosophila
  • beta-Galactosidase