Peculiarities of receptor-channel complexes for inhibitory mediators in the membranes of lamprey spinal cord neurones

Neurosci Lett. 1989 Jul 17;102(1):82-6. doi: 10.1016/0304-3940(89)90311-x.

Abstract

Effects of inhibitory mediators on the membranes of isolated lamprey spinal cord neurones were investigated by means of whole-cell recording and concentration clamp techniques. Glycine and gamma-aminobutyric acid (GABA) applications evoked desensitizing chloride currents. The concentrations at half-maximum effects were 16 microM for glycine- and 1.5 mM for GABA-activated currents. Ionic responses to applications of both amino acids demonstrated full cross-desensitization. Strychnine and bicuculline suppressed both glycine- and GABA-activated conductances to the same degree. We suggest that in the membranes of lamprey spinal cord neurones glycine and GABA act on the same receptor-channel complexes.

MeSH terms

  • Animals
  • Bicuculline / pharmacology
  • Fishes / physiology*
  • Glycine / pharmacology*
  • In Vitro Techniques
  • Lampreys / physiology*
  • Neural Inhibition / drug effects*
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / physiology*
  • Spinal Cord / drug effects
  • Spinal Cord / physiology*
  • Strychnine / pharmacology
  • gamma-Aminobutyric Acid / pharmacology*

Substances

  • Receptors, Neurotransmitter
  • gamma-Aminobutyric Acid
  • Strychnine
  • Glycine
  • Bicuculline