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. 2014 Dec 12;3(4):920-33.
doi: 10.3390/pathogens3040920.

Proteomic Differences between Listeria monocytogenes Isolates from Food and Clinical Environments

Ge Huang  1 , Susan L Mason  2 , J Andrew Hudson  3 , Stefan Clerens  4 , Jeffrey E Plowman  5 , Malik A Hussain  6
Affiliations

Proteomic Differences between Listeria monocytogenes Isolates from Food and Clinical Environments

Ge Huang et al. Pathogens. .

Abstract

Listeria monocytogenes is an organism associated with a wide range of foods. It causes listeriosis, a severe illness that mainly affects people with weakened immune systems. Proteomic profiles of three different L. monocytogenes isolates were studied using 1D SDS PAGE, 2DE and mass spectrometry. The protein banding patterns generated by 1D SDS PAGE of three strains of L. monocytogenes were found to be similar. Visual observations from 2DE gel maps revealed that certain spots appeared to have intensity differences. Key differences in proteins synthesis of three strains of L. monocytogenes were found using the PDQest TM 2DE Analysis software. Comparison showed that the clinical isolate (strain SB92/844) had 53.4% and 53.9% protein profile similarity with dairy isolate (strain V7) and seafood isolate (SB92/870), respectively. The identity of selected protein spots was achieved using MALDI-TOF and ion trap mass spectrometry. It was found that certain identified proteins (i.e., a major cold shock protein and superoxide dismutase) were expressed differently between two local strains of L. monocytogenes (SB92/844, SB92/870) and one strain from overseas (V7).

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Figures

Figure 1
Figure 1
Growth of three strains of L. monocytogenes (V7, SB92/870, SB92/844) in brain heart infusion (BHI) medium at 37 °C (A) until the bacterial growth reached the stationary phase; (B) 1D SDS-PAGE gel of the three strains of L. monocytogenes showing the banding pattern of each strain. Lanes 1 and 5, protein marker; Lane 2, strain SB92/844; Lane 3, strain SB92/870; Lane 4, strain V7.
Figure 2
Figure 2
2DE gels maps of three strains of Listeria monocytogenes to indicate some of the differences in protein spots observed visually. (e.g., Spot Group 1, Spot 2, Spot 3, Spot 4 and Spot 5).
Figure 3
Figure 3
Graphical presentation of the number of differentially expressed protein spots between the strains. Data acquired through spot analysis using PDQuest (v 7.0.0) software.
Figure 4
Figure 4
A 2DE map of L. monocytogenes strain SB92/844.
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