Objective: To determine whether endometriosis is associated with mitochondrial dysfunction in cumulus (granulosa [GC]) cells of subjects undergoing IVF-intracytoplasmic sperm injection (ICSI).
Design: Prospective cohort study.
Setting: An IVF clinic in a tertiary academic care center.
Patient(s): Eleven women with endometriosis and 39 controls.
Main outcome measure(s): Cumulus cell adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA), and genomic DNA (gDNA) number.
Result(s): Cumulus cell ATP content was 65% lower in subjects with surgically proven endometriosis (median 312.5 attomoles/ng total DNA, interquartile range = 116.0-667.8) compared with controls (median 892.4 attomoles/ng total DNA, interquartile range = 403.0-1,412.2). There was no significant difference in mtDNA:gDNA ratio. There were no significant differences in age, body mass index (BMI), basal serum FSH level, total oocyte number, metaphase II (M2) oocyte number, metaphase I oocyte number, percentage of M2 oocytes, fertilization rate, implantation rate, or pregnancy rate (PR). Multivariate regression analysis showed significant positive correlations between ATP and  M2 oocyte number (r = 0.307) and  pregnancy (r = 0.332). There were also trends toward positive correlations between ATP and  age (r = 0.283),  total number of oocytes (r = 0.271),  percentage of M2 oocytes (r = 0.249), and  implantation rate (r = 0.293). There were no statistically significant correlations between mtDNA:gDNA ratio and any demographic factors or clinical outcomes measured.
Conclusion(s): Surgically confirmed endometriosis may be associated with cumulus cell mitochondrial dysfunction in subjects undergoing IVF-ICSI for infertility, as reflected by decreased ATP production.
Keywords: Endometriosis; cumulus cells; infertility; mitochondria.
Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.