Influence of surfaces modified with biomimetic extracellular matrices on adhesion and proliferation of mesenchymal stem cells and osteosarcoma cells

Rong Cai; Naoki Kawazoe; Guoping Chen

doi:10.1016/j.colsurfb.2014.11.050

Influence of surfaces modified with biomimetic extracellular matrices on adhesion and proliferation of mesenchymal stem cells and osteosarcoma cells

Colloids Surf B Biointerfaces. 2015 Feb 1:126:381-6. doi: 10.1016/j.colsurfb.2014.11.050. Epub 2014 Dec 9.

Authors

Rong Cai¹, Naoki Kawazoe², Guoping Chen³

Affiliations

¹ Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
² Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
³ Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: Guoping.Chen@nims.go.jp.

PMID: 25516267
DOI: 10.1016/j.colsurfb.2014.11.050

Abstract

Preparation of surfaces modified with biomimetic extracellular matrices (ECMs) is important for investigation of the interaction between ECMs and cells. In the present study, surfaces modified with ECMs from normal somatic cells, stem cells and tumor cells were prepared by cell culture method. The ECMs derived from bone marrow-derived mesenchymal stem cells (MSCs), dermal fibroblasts (FBs), osteoblasts (OBs) and MG63 osteosarcoma cells were deposited on the surfaces of cell-culture polystyrene plates (TCPS). The ECMs from different cell types had different compositions. The effects of the ECM-deposited surfaces on the adhesion, spreading and proliferation of MSCs and MG63 human osteosarcoma cells were dependent on the type of both ECMs and cells. The surfaces deposited with ECMs from MSCs, FBs and OBs promoted cell adhesion more strongly than surfaces deposited with ECMs from MG63 cells and TCPS. Compared to TCPS, the ECM-deposited surfaces promoted proliferation of MSCs while they inhibited the proliferation of MG63 cells.

Keywords: Biomimetic surface; Extracellular matrices; Stem cells; Surface modification; Tumor cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomimetic Materials / chemistry*
Cell Adhesion
Cell Proliferation
Cells, Cultured
Extracellular Matrix / chemistry*
Humans
Mesenchymal Stem Cells / cytology*
Osteosarcoma / pathology*
Particle Size
Surface Properties