Customizing properties of β-chitin in squid pen (gladius) by chemical treatments

Mar Drugs. 2014 Dec 15;12(12):5979-92. doi: 10.3390/md12125979.


The squid pen (gladius) from the Loligo vulgaris was used for preparation of β-chitin materials characterized by different chemical, micro- and nano-structural properties that preserved, almost completely the macrostructural and the mechanical ones. The β-chitin materials obtained by alkaline treatment showed porosity, wettability and swelling that are a function of the duration of the treatment. Microscopic, spectroscopic and synchrotron X-ray diffraction techniques showed that the chemical environment of the N-acetyl groups of the β-chitin chains changes after the thermal alkaline treatment. As a consequence, the crystalline packing of the β-chitin is modified, due to the intercalation of water molecules between β-chitin sheets. Potential applications of these β-chitin materials range from the nanotechnology to the regenerative medicine. The use of gladii, which are waste products of the fishing industry, has also important environmental implications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chitin / chemistry*
  • Decapodiformes / chemistry*
  • Microscopy / methods
  • Nanotechnology / methods
  • Porosity
  • Spectrum Analysis / methods
  • Water / chemistry
  • Wettability
  • X-Ray Diffraction / methods


  • Water
  • Chitin