Coxsackievirus B3 myocarditis in C3H/HeJ mice: description of an inbred model and the effect of exercise on virulence

Eur J Epidemiol. 1989 Sep;5(3):348-50. doi: 10.1007/BF00144836.


The effect of forced exercise on the development of coxsackievirus B3 myocarditis in inbred C3H/HeJ mice was studied. Four groups of mice (30 per group) were formed: infected-exercised (Group I); infected-unexercised (Group II); uninfected-exercised (Group III); and uninfected-unexercised (Group IV). Infected mice were inoculated intraperitoneally with 1.0 x 10(2.1) TCID50 coxsackievirus B3. Exercised animals were swum daily for 60 minutes on days 1-9. Myocardial viral titers were acutely elevated on day 3 of infection and were augmented significantly by exercise on days 6 and 9. Exercise increased the overall mortality from 0-10% to 20-40%; significantly increased heart: body weight ratios on days 6, 9 and 13; and increased the extent of myocardial fiber necrosis. We have reproduced the acceleration of CB3 myocarditis by exercise in the inbred C3H model.

MeSH terms

  • Animals
  • Body Weight
  • Coxsackievirus Infections* / immunology
  • Coxsackievirus Infections* / mortality
  • Coxsackievirus Infections* / pathology
  • Disease Models, Animal*
  • Enterovirus B, Human / immunology
  • Enterovirus B, Human / pathogenicity*
  • Male
  • Mice
  • Mice, Inbred C3H / immunology
  • Mice, Inbred C3H / microbiology*
  • Myocarditis / etiology*
  • Myocarditis / mortality
  • Myocarditis / pathology
  • Myocardium / pathology
  • Organ Size
  • Physical Exertion*
  • Swimming
  • Virulence