Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

ACS Nano. 2015 Jan 27;9(1):251-9. doi: 10.1021/nn504508s. Epub 2014 Dec 18.


Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.

Keywords: FRET; RNA and DNA nanoparticles; RNA and DNA nanotechnology; RNAi; RNA−DNA hybrids reassociation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • DNA / chemistry*
  • Drug Carriers / chemistry*
  • HEK293 Cells
  • HIV-1 / genetics
  • Humans
  • Models, Molecular
  • Nanoparticles / chemistry*
  • Nucleic Acid Conformation
  • RNA / chemistry*
  • RNA Interference*
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Ribonuclease III / metabolism


  • Drug Carriers
  • RNA, Small Interfering
  • RNA
  • DNA
  • Ribonuclease III