Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract

Mol Nutr Food Res. 2015 Mar;59(3):424-33. doi: 10.1002/mnfr.201400674. Epub 2015 Jan 22.

Abstract

Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults.

Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ∼3 x higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21).

Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.

Keywords: Absorption; Broccoli sprout; Chemoprevention; Excretion; Sulforaphane.

Publication types

  • Randomized Controlled Trial
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Anticarcinogenic Agents / pharmacokinetics
  • Anticarcinogenic Agents / pharmacology*
  • Brassica / chemistry*
  • Dietary Supplements
  • Gene Expression Regulation / drug effects
  • Glycoside Hydrolases / chemistry*
  • Heme Oxygenase-1 / blood
  • Heme Oxygenase-1 / genetics
  • Histone Deacetylases / blood
  • Humans
  • Intestinal Absorption
  • Isothiocyanates / administration & dosage
  • Isothiocyanates / pharmacokinetics
  • Isothiocyanates / pharmacology*
  • Middle Aged
  • Molecular Targeted Therapy / methods
  • Plant Extracts / pharmacology
  • Sulfoxides
  • Young Adult

Substances

  • Anticarcinogenic Agents
  • Isothiocyanates
  • Plant Extracts
  • Sulfoxides
  • HMOX1 protein, human
  • Heme Oxygenase-1
  • Glycoside Hydrolases
  • thioglucosidase
  • Histone Deacetylases
  • sulforaphane