Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli

Mol Microbiol. 1989 Sep;3(9):1173-82. doi: 10.1111/j.1365-2958.1989.tb00267.x.

Abstract

Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane-derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5 kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA+ plasmid produced three to four times more MDO than wild-type cells. MDO overproduction did not affect the degree of MDO substitution with sn-1-phosphoglycerol residues. The biosynthesis of MDO remained under osmotic control in overproducing strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • Conjugation, Genetic
  • DNA Transposable Elements
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Membranes / metabolism
  • Mutation
  • Oligosaccharides / biosynthesis*
  • Oligosaccharides / genetics
  • Osmotic Pressure
  • Plasmids
  • Restriction Mapping
  • Transduction, Genetic

Substances

  • DNA Transposable Elements
  • Oligosaccharides