Antioxidative capacity of a dietary supplement on retinal hemodynamic function in a human lipopolysaccharide (LPS) model

Invest Ophthalmol Vis Sci. 2014 Dec 18;56(1):403-11. doi: 10.1167/iovs.14-15581.


Purpose: Beneficial effects of dietary supplements in age-related macular degeneration (AMD) are related to antioxidative properties. In the Age-Related Eye Disease Study 1 (AREDS 1), a reduced progression to late stage AMD was found using vitamin C, E, zinc, and β-carotene. We showed previously that the AREDS 1 formulation restores the O2-induced retinal vasoconstrictor response of retinal vessels in a human endotoxin (lipopolysaccharide [LPS]) model.

Methods: We hypothesized that the abnormal O2-induced retinal red blood cell (RBC) flow response can be modulated by a different formulation (vitamin C, E, and zinc, lutein/zeaxanthin, selenium, taurine, Aronia extract, and omega-3 free fatty acids). A total of 43 healthy subjects was included in this randomized, double masked, placebo-controlled parallel group study. The reactivity of retinal arterial and venous diameter, RBC velocity, and flow to 100% O2 breathing was investigated in the absence and presence of 2 ng/kg LPS. Between the two study days was a 14-day period of daily dietary supplement intake.

Results: The decrease in retinal arterial diameter, RBC velocity, and flow during 100% O2 breathing was diminished significantly after LPS infusion. Dietary supplement intake for 14 days almost restored the response of retinal hemodynamic parameters to 100% O2 after LPS administration. This effect was significant for retinal arterial diameter (P = 0.03 between groups), and RBC velocity and flow (each P < 0.01 between groups).

Conclusions: The present data indicate restoring of the RBC flow response to 100% O2 after LPS administration. This is likely due to an amelioration of endothelial dysfunction resulting from oxidative stress, a factor involved in AMD pathophysiology. ( number, NCT00914576.).

Keywords: dietary supplements; endotoxin model; red blood cell flow; retinal blood flow; retinal hemodynamic function; white blood cell flow; young healthy subjects.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antioxidants / administration & dosage*
  • Dietary Supplements*
  • Double-Blind Method
  • Female
  • Follow-Up Studies
  • Healthy Volunteers
  • Hemodynamics / drug effects
  • Hemodynamics / physiology*
  • Humans
  • Laser-Doppler Flowmetry
  • Lipopolysaccharides / adverse effects
  • Macular Degeneration / chemically induced
  • Macular Degeneration / physiopathology
  • Macular Degeneration / prevention & control*
  • Male
  • Regional Blood Flow
  • Retina / drug effects
  • Retina / physiopathology*
  • Retinal Vessels / drug effects
  • Retinal Vessels / physiopathology
  • Treatment Outcome
  • Young Adult


  • Antioxidants
  • Lipopolysaccharides
  • lipopolysaccharide A

Associated data