Distribution of 8 periodontal microorganisms in family members of Chinese patients with aggressive periodontitis

Arch Oral Biol. 2015 Mar;60(3):400-7. doi: 10.1016/j.archoralbio.2014.11.015. Epub 2014 Dec 2.

Abstract

Objective: To date, no information on the distribution of periodontal microorganisms among family members of Chinese patients with aggressive peridontitis (AgP) is available. The aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members.

Design: Saliva and pooled subgingival plaque samples were collected from 103 participants from 41 nuclear families (including 41 AgP probands, 19 mothers, 22 fathers, 21 siblings). Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis-positive individuals by PCR.

Results: P. gingivalis, T. forsythia, T. denticola, C. rectus and F. nucleatum were the most frequently detected species both in AgP probands and in their relatives. Kappa statistical analysis revealed that the detection of A. actinomycetemcomitans (Kappa = 0.503) and F. nucleatum (Kappa = 0.565) in probands was highly consistent with that in their relatives. Most probands shared the identical fimA genotype of P. gingivalis with their relatives.

Conclusions: Our results suggested that the intrafamilial transmission of periodontal microorganisms may occur between Chinese patients with aggressive periodontitis and their relatives.

Keywords: Aggressive periodontitis; Genotype; Microbiology; Saliva.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggregatibacter actinomycetemcomitans / isolation & purification
  • Aggressive Periodontitis / microbiology*
  • Bacteroidetes / isolation & purification
  • Campylobacter rectus / isolation & purification
  • China
  • Dental Plaque / microbiology
  • Electrophoresis, Agar Gel
  • Female
  • Fusobacterium nucleatum / isolation & purification
  • Genotype
  • Humans
  • Male
  • Nuclear Family*
  • Polymerase Chain Reaction
  • Porphyromonas gingivalis / isolation & purification
  • Prevotella intermedia / isolation & purification
  • Prevotella nigrescens / isolation & purification
  • Saliva / microbiology
  • Treponema denticola / isolation & purification