Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC-MS/MS

Bioanalysis. 2014;6(23):3081-9. doi: 10.4155/bio.14.254.

Abstract

Aim: Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume.

Results: We report a sensitive LC-MS/MS method for quantitation of PQ with only 25 µl human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI(+) as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5-250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children.

Conclusion: The developed LC-MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analytic Sample Preparation Methods
  • Antimalarials / blood*
  • Blood Chemical Analysis / methods*
  • Child
  • Chromatography, Liquid
  • Clinical Trials as Topic
  • Humans
  • Quinolines / blood*
  • Tandem Mass Spectrometry*

Substances

  • Antimalarials
  • Quinolines
  • piperaquine