The galactolipase activity of Fusarium solani (phospho)lipase

Biochim Biophys Acta. 2015 Mar;1851(3):282-9. doi: 10.1016/j.bbalip.2014.12.010. Epub 2014 Dec 18.


The purified (phospho)lipase of Fusarium solani (FSL), was known to be active on both triglycerides and phospholipids. This study aimed at assessing the potential of this enzyme in hydrolyzing galactolipids. FSL was found to hydrolyze at high rates of synthetic medium chains monogalactosyldiacylglycerol (4658±146U/mg on DiC8-MGDG) and digalactosyldiacylglycerol (3785±83U/mg on DiC8-DGDG) and natural long chain monogalactosyldiacylglycerol extracted from leek leaves (991±85U/mg). It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. FSL maximum activity on galactolipids was measured at pH8. The analysis of the hydrolysis product of natural MGDG from leek showed that FSL hydrolyzes preferentially the ester bond at the sn-1 position of galactolipids. To investigate the structure-activity relationships of FSL, a 3D model of this enzyme was built. In silico docking of medium chains MGDG and DGDG and phospholipid in the active site of FSL reveals structural solutions which are in concordance with in vitro tests.

Keywords: Fusarium solani (phospho)lipase; Galactolipase; Galactolipid; In silico docking; Leek.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxylic Ester Hydrolases / chemistry*
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / metabolism
  • Catalytic Domain
  • Enzyme Assays
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Fusarium / chemistry*
  • Fusarium / enzymology
  • Galactolipids / chemical synthesis
  • Galactolipids / chemistry
  • Galactolipids / isolation & purification
  • Gene Expression
  • Humans
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Kinetics
  • Lipase / chemistry
  • Molecular Docking Simulation
  • Molecular Dynamics Simulation
  • Onions / chemistry
  • Phospholipases / chemistry*
  • Phospholipases / genetics
  • Phospholipases / metabolism
  • Plant Leaves / chemistry
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Substrate Specificity


  • Fungal Proteins
  • Galactolipids
  • Recombinant Proteins
  • digalactosyldiacylglycerol
  • monogalactosyldiacylglycerol
  • Phospholipases
  • Carboxylic Ester Hydrolases
  • galactolipase
  • Lipase
  • pancreatic lipase related protein 2