[Retinoic acid modification of cell culture used for reproduction of enteropathogenic viruses]

Biull Eksp Biol Med. 1989 Jul;108(7):93-5.
[Article in Russian]


The 0.001-0.005% retinoic acid injection into the growth cultural medium of prime and continue cell cultures 12-24h before inoculation considerably raised the cell sensitivity to animal entero- and coronaviruses. The entero- and coronaviruses concentrations in cultural medium increased by 10(1.58) and 10(1.68)TCID 50/1.0 respectively. The optimized parameters of the cell culture processing for the enteropathogenic viruses reproduction improvement are proposed.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Cells, Cultured
  • Coronaviridae / growth & development*
  • Culture Media*
  • Enterovirus / growth & development*
  • Enteroviruses, Porcine / growth & development*
  • Swine
  • Time Factors
  • Transmissible gastroenteritis virus / growth & development*
  • Tretinoin / pharmacology*
  • Virus Cultivation


  • Culture Media
  • Tretinoin