A second-generation phosphohistidine analog for production of phosphohistidine antibodies

Org Lett. 2015 Jan 16;17(2):187-9. doi: 10.1021/ol503320p. Epub 2014 Dec 22.

Abstract

Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Phospho-Specific / chemistry*
  • Histidine / analogs & derivatives*
  • Histidine / chemistry
  • Humans
  • Molecular Structure
  • Phosphorylation
  • Proteins / chemistry*
  • Pyrazoles / chemical synthesis*
  • Pyrazoles / chemistry
  • Signal Transduction
  • Triazoles / chemical synthesis*
  • Triazoles / chemistry

Substances

  • Antibodies, Phospho-Specific
  • Proteins
  • Pyrazoles
  • Triazoles
  • Histidine
  • phosphohistidine