Cardioprotective Effect of Hydrogen-rich Saline on Isoproterenol-induced Myocardial Infarction in Rats

Heart Lung Circ. 2015 Jun;24(6):602-10. doi: 10.1016/j.hlc.2014.11.018. Epub 2014 Dec 4.


Background: Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant and anti-inflammatory activity that may protect against organ damage induced by oxidative stress. Therefore because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of our study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats.

Methods: An acute MI model was induced in male Wistar rats by subcutaneous injection of isoproterenol. Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats. Oxidative stress indices including levels of myocardial marker enzymes, inflammatory cytokines, membrane-bound myocardial enzymes and histopathological changes were measured.

Results: Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished infarct size, improved left heart function, and ameliorated pathological changes of the left heart.

Conclusion: From these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities.

Keywords: Hydrogen; Inflammation; Isoproterenol; Myocardial infarction; Oxidative stress.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Biomarkers / blood
  • Biopsy, Needle
  • Cardiotonic Agents / pharmacology*
  • Cytokines / blood
  • Disease Models, Animal
  • Drug Combinations
  • Hydrogen / pharmacology*
  • Immunohistochemistry
  • Interleukin-6 / blood
  • Isoproterenol / pharmacology*
  • Male
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / pathology*
  • Oxidative Stress
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reference Values
  • Sodium Chloride / pharmacology*
  • Tumor Necrosis Factor-alpha / blood


  • Biomarkers
  • Cardiotonic Agents
  • Cytokines
  • Drug Combinations
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Sodium Chloride
  • Hydrogen
  • Isoproterenol