Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production

C J McNeil; K A Smith; P Bellavite; J V Bannister

doi:10.3109/10715768909087928

Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production

Free Radic Res Commun. 1989;7(2):89-96. doi: 10.3109/10715768909087928.

Authors

C J McNeil¹, K A Smith, P Bellavite, J V Bannister

Affiliation

¹ Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, England.

PMID: 2553552
DOI: 10.3109/10715768909087928

Abstract

An electrochemical sensor for the measurement of the superoxide anion produced as a result of the respiratory burst of neutrophils is described. The reduction of cytochrome c by the superoxide ion was monitored using a surface-modified gold electrode. The current produced was shown to be superoxide-specific and proportional to the number of neutrophils in both purified neutrophil preparations and plasma.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Cytochrome c Group / metabolism*
Electrochemistry
Electrodes
Free Radicals
Humans
In Vitro Techniques
Neutrophils / metabolism
Spectrophotometry
Superoxides / metabolism*
Xanthine
Xanthine Oxidase / metabolism
Xanthines / metabolism

Substances

Cytochrome c Group
Free Radicals
Xanthines
Superoxides
Xanthine
Xanthine Oxidase