The suspension culture of undifferentiated human pluripotent stem cells using spinner flasks

Methods Mol Biol. 2015;1283:13-21. doi: 10.1007/7651_2014_118.


Suspension cell culture systems with superior scalability, controllability, and monitoring options are an attractive alternative to static adherent culture methods for expansion and production of human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent cells (iPSCs). In this chapter, we describe a scalable suspension culture system using serum-free, feeder-free, matrix-free, and defined culture conditions with spinner flasks for hPSC maintenance and expansion. This suspension culture system provides an efficient and GMP-compatible process for large-scale manufacture of hPSCs.

MeSH terms

  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cryopreservation / methods
  • Embryonic Stem Cells / cytology
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / cytology*