L-type calcium channels in the regulation of neurite outgrowth from rat dorsal root ganglion neurons in culture

Neurosci Lett. 1989 Sep 25;104(1-2):110-4. doi: 10.1016/0304-3940(89)90338-8.

Abstract

Dorsal root ganglion neurons from neonatal rats were grown in culture for 12 h and the extent of neurite outgrowth determined by counting the fraction of neurons with neurites. In the presence of high K+, veratridine or bradykinin the extent of neurite outgrowth was reduced by about 60%. The inhibitory effect of depolarisation was reversible and was abolished by nifedipine. gamma-Aminobutyric acid (GABA), baclofen and 2-chloroadenosine had no effect on neurite outgrowth in control cultures but abolished the inhibitory effect of depolarisation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Chloroadenosine / pharmacology
  • Animals
  • Animals, Newborn
  • Axons / drug effects
  • Axons / physiology*
  • Baclofen / pharmacology
  • Bradykinin / pharmacology
  • Calcium Channels / physiology*
  • Cells, Cultured
  • Ganglia, Spinal / ultrastructure*
  • Neurons / ultrastructure*
  • Potassium / pharmacology
  • Rats
  • Veratridine / pharmacology
  • gamma-Aminobutyric Acid / pharmacology

Substances

  • Calcium Channels
  • 2-Chloroadenosine
  • gamma-Aminobutyric Acid
  • Veratridine
  • Baclofen
  • Potassium
  • Bradykinin