The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model

Biomaterials. 2015 Feb:42:87-93. doi: 10.1016/j.biomaterials.2014.11.037. Epub 2014 Dec 15.


Postoperative regeneration of the middle ear mucosa and pneumatization of the middle ear cavity are of great importance after middle ear surgery. This study developed a new method to transplant autologous nasal mucosal epithelial cell sheets into the damaged middle ear cavity. The aim of this study was to evaluate postoperative healing after the transplantation of the cell sheets. Rabbit nasal mucosal epithelial cell sheets were fabricated on a temperature-responsive culture dish, and transplanted into the damaged middle ear of rabbit, which was surgically created. The healing of middle ears was evaluated by histology and X-ray computed tomography after transplantation. Functional evaluation was performed by measuring the maximum middle ear total pressure reflecting a trans-mucosal gas exchange function. Two control groups were used: the normal control group and the mucosa-eliminated control group. Transplantation of cell sheets suppressed the bone hyperplasia and the narrowing of pneumatic space in the middle ear cavity compared with the mucosa-eliminated control group. The mucosal gas exchange function was also better in the cell sheet-transplanted group. Nasal mucosal epithelial cell sheet was confirmed to be useful as an effective graft material after middle ear surgery and hopefully become a novel therapy in the future.

Keywords: Cell sheet; Cholesteatoma; Middle ear surgery; Mucosal regeneration; Nasal mucosal epithelium; Temperature-responsive culture dish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Ear, Middle / diagnostic imaging
  • Ear, Middle / surgery*
  • Epithelial Cells / cytology
  • Epithelial Cells / transplantation*
  • Immunohistochemistry
  • Nasal Mucosa / cytology*
  • Pressure
  • Rabbits
  • Temporal Bone / diagnostic imaging
  • Tomography, X-Ray Computed